News: 2011 bioblitz!

bioblitz shieldFarrell Lab undergraduate Adam Clark is leading the second annual Harvard bioblitz. Join us on May 1! More

News

Prof. Farrell co-authors new paper which answers longstanding scientific question about cause of tropics' stunning biodiversity. More

Current research

News pictureCurrent research extends the evolution of insect-plant interactions to other trophic levels through a broad collaboration in the beetle Tree of Life project.

A new research dimension in the lab concerns the acoustic signals produced for mating and territory defense. More

Techniques: PCR - Ordering & suspending primers

We order primers from Integrated DNA Technologies. When resuspending them for storage, we want them to have a concentration of 100 µM (micromolar). To calculate how much TE buffer to suspend in, multiply the ODU on the primer tube by the molarity of the solution one would get by suspending 1 OD of the oligo in 1.0 ml (should be written on the paper accompanying the primer). Divide this number by 100 to determine the amount of TE to suspend the primer in. For example, if the tube says 17 ODU, and the paper says "1 OD of this oligo in 1.0 ml volume will yield a 4.3 µmolar solution", use 0.731 ml or 731 µl of TE. When aliquoting into working primer tubes, dilute 10:1 with water. Alternatively, you can use IDT's nifty resuspension calculator